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. Author manuscript; available in PMC: 2009 Mar 9.
Published in final edited form as: Eur J Immunol. 2006 Jun;36(6):1374–1385. doi: 10.1002/eji.200635883

Figure 5.

Figure 5

Activation of the STAT1 pathway and expression of T-bet, IL-2 and IL-10 in naive and PI-Treg cells in the presence of IL-2. CD4+ T cells were isolated from the spleens of naive and tolerant mice 2 h before and after antigen stimulation in vivo. Total cellular lysates were analyzed for STAT1 activation by phospho-STAT1-specific antibody (A). In addition, the naive and PI-Treg cells purified before antigen stimulation were treated in vitro with or without IL-2 (10 U/mL) for 24 h. The T cells were isolated by anti-CD4 antibody-coated beads and submitted to semiquantitative PCR for IL-2, IL-10 and T-bet (B). β2 m served as loading control. These results were consistent in three separate experiments.