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Effect of DA on simultaneous release of GnRH and cAMP from GT1 cells. GT1 cell-coated coverslips were perifused with Locke's medium and samples were collected every 4 min after a 1-h equilibration period. DA (1 μM) was added to the perifused medium for the second hour of collection. RIA was used to measure both cAMP and GnRH in the samples.
