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Effect of blockade of PKA activity on GnRH release. Treatment with H-89 (10 μM) had no effect on stimulation of GnRH release by addition of 8-Br-cAMP (1 mM) and DA (1 μM) to GT1 cells. However, in both experiments treatment with H-89 alone stimulated basal GnRH release. Each point represents the mean ± SE of six wells.
