Table 1.

Histopathological scoring system for lung, liver and spleen of F. tularensis SchuS4 infected mice.

Lung		Liver	Spleen
A	Peribronchial and bronchial infiltrates (% of sites)	Hepatic lobules infiltration	Marginal zone thickening and inflammation
	0 = None	0 = None	0 = None
	1 = Few (<25%)	1 = Few neutrophils in sinuses	1 = Mild
	2 = Many (25–75%)	2–3 = Many neutrophils and engorgement	2 = Moderate
	3 = All (>75%)		3 = Severe
B	Inflammatory infiltrates	Quantity of granulomas (10 × magnification) and distribution	Perilymphoid red pulp infiltration
	0 = None	0 = None	0 = None
	1 = Mild (interrupted)	1= Few (1–5)	1 = Mild
	2 = Moderately complete (Collar <5 cells)	2 = Moderate (5–10)	2 = Moderate
	3 = Severe (collar >5–10 cells)	3 = Many (>10)	3 = Severe
C	Quality of infiltrate	Quality of granulomatous infiltrates	Red pulp parenchymal inflammation
	0 = None	0 = None	0 = None
	1 = Mild neutrophilic	1 = Non discrete (mild neutrophilic)	1 = Mild
	2 = Moderate neutrophilic	2 = Discrete (more neutrophilic and lymphocytic)	2 = Moderate
	3 = macrophages and neutrophils	3 = Severe and necrotic	3 = Severe
	4 = Macrophages only
D	Parenchymal pneumonia	Portal triad infiltration	Quality of granulomatous inflammation
	0 = None	0 = None	0 = None
	1 = Minimal (patchy)	1 = Mild neutrophilic	1 = Mild
	2 = Heavy (patchy)	2 = Moderate neutrophilic	2 = Moderate
	3 = Heavy, confluent and necrotizing	3 = Severe (macrophages and neutrophils)	3 = Necrotizing
E	Bronchiolar and bronchiole lumen exudates
	0 = None
	1 = Minimal (25% lumen occlusion)
	2 = Heavy (>25%)
F	Perivascular infiltrate
	0 = None
	1 = Mild
	2 = Moderate (10–50%)
	3 = Heavy (>50% blood vessels involved)
Total Score	18	12	12