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. 2009 Mar 11;4(3):e4809. doi: 10.1371/journal.pone.0004809

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Heatmap showing 134 Pdu peptides that represent 11 individual Pdu proteins up-regulated under acidic minimal media (AMM-2) growth condition (Panel A) and 124 Pdu peptides that represent 11 individual Pdu proteins up-regulated under LB Stat growth condition (Panel D) in Δhfq and ΔsmpB strains relative to wild-type. Salmonella strains harboring HA-tag at each Pdu gene were grown in acidic minimal media (AMM-2) (Panel B and C) and under LB stat phase (Panel E and F) as described. Same amount of cell lysates was loaded in each lane and probed by Western blot analysis for the Pdu-encoded proteins and a control protein DnaK. The level of Pdu-encoded protein expressed in each strain was normalized to the DnaK level. Intracellular DnaK level in each lane was set to 100%.