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. 2009 Mar 11;4(3):e4809. doi: 10.1371/journal.pone.0004809

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Total RNA was isolated from Salmonella cells grown under acidic minimal media (AMM-2) growth condition (Panel A) or LB Stat growth condition (Panel B) and mRNA level of pdu genes was quantified via RT-PCR using gyrB mRNA level for normalization. As controls the mRNA level of smpB and hfq genes were also quantified in a similar fashion. Expression ratios (smpB or hfq/14028s) were averaged from three individual experiments and shown on a logarithmic scale.