Fig. 2.
Processing pro-ANP by corin variants. A. Human pro-ANP was expressed in HEK 293 cells and then added to the cells expressing wild-type corin and variants. Pro-ANP processing was analyzed (top panel). Corin protein expression in the transfected cells was verified by Western blotting (lower panel). B. Pro-ANP processing activities of corin variants. Pro-ANP processing activity was quantified by densitometric analysis of Western blots. The data represent mean ± S.D. from six independent experiments. **p<0.01 vs. WT. n.s., not statistically significant vs. WT.