Table 1.
Primer Sequences and Products of RT-PCR
| Gene | GenBank # | Forward Primer | Reverse Primer | Size of PCR product |
|---|---|---|---|---|
| Elastin | NM_000501 | 5’-AAGCAGCAGCAAAGTTCGGT-3’ | 5’-ACTAAGCCTGCAGCAGCTCCATA-3’ | 187bp |
| Fibronectin | NM_002026 | 5’-ACCTACGGATGACTCGTGCTTTGA-3’ | 5’-CAAAGCCTAAGCACTGGCACAACA-3’ | 116bp |
| Procollagen I α-1 | NM_000088 | 5’-AATGGTGCTCCTGGTATTGCTGGT-3’ | 5’-ACCAGTGTCTCCTTTGCTGCCA-3’ | 141bp |
| Collagen I α -2 | NM_000089 | 5’-AACAAATAAGCCATCACGCCTGCC-3’ | 5’-TGAAACAGACTGGGCCAATGTCCA- 3’ | 101bp |
| Collagen VI α -3 | NM_057167 | 5’-GAAGAAGGAGTCAGCCATCG-3’ | 5’-ATTCGAGAGAACTGCCTGGA-3’ | 189bp |
| β-Actin | NM_001101 | 5’-ACGTTGCTATCCAGGCTGTGCTAT-3’ | 5’-CTCGGTGAGGATCTTCATGAGGTAGT-3’ | 188bp |