Skip to main content
. 2009 Mar 2;6:21. doi: 10.1186/1742-4690-6-21

Table 2.

Primers and probes used to quantify viral sequences by real-time PCR

GAPDH
forward primer 5'- ACCCCTGGCCAAGGTCATC
reverse primer 5'- AGGGGCCATCCACAGTCTTC
Probe 5'- (6-Fam)AGGACTCATGACCACAGTCCATGCCA(Tamra)
CypA
forward primer 5'- GGCCGCGTCTCCTTTGA
reverse primer 5'- AATCCTTTCTCTCCAGTGCTCAGA
Probe 5'- (6-Fam)TGCAGACAAGGTCCCAAAGACAGCAG(Tamra)

TRIM5α
forward primer 5'- TGCCTCTGACACTGACTAAGAAGATG
reverse primer 5'- GGGCTAAGGACTCATTCATTGG
Probe 5'- (6-Fam)AAGCTTTTCAACAGCCTTTCTATATCATCGTGTGATA(Tamra)