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SDS-PAGE analysis of constructed mutants and wild-type CotA. Whole cells and cell lysates of the CotA mutants D500G (D), K316N (K), and K316N/D500G (DK) as well as of wild-type CotA (CotA) expressed overnight in E. coli BL21(DE3) cells; molecular size marker from Fermentas (M).
