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Homology model of K316N/D500G (based on 1GSK [25]). A) Amino acid residue asparagine 316 is shown in yellow; glycine 500 is shown in red. The T1 copper and the trinuclear copper cluster are highlighted in orange. B) Superposition of the catalytic center of wild-type CotA and K316N/D500G with Asp500 shown in yellow, Gly500 shown in red, the axial T1 ligand Met501 shown in green, and Met502 shown in blue. The T1 copper and the trinuclear copper cluster are highlighted in orange.
