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. Author manuscript; available in PMC: 2009 Dec 31.
Published in final edited form as: J Am Chem Soc. 2008 Dec 31;130(52):17801–17811. doi: 10.1021/ja8054035

Figure 5.

Figure 5

Stopped-flow fluorescence quenching by the anaerobic addition of 48 Fe2+ to apo-variant #3 (curve a), of 12 Fe2+ to apo-variant #1 with 8 Zn2+ bound (curve b), of 12 Fe2+ to apo-variant #1 (curve c), and of 48 Fe2+ to apo-variant #1 (curve d). Each curve is the average of 6 to 9 runs. Final conditions: 0.5 μM protein in 25 mM Mops hemisodium salt, 12.5 mM NaCl, pH 7.15, 25 °C. The Fe2+ solution was prepared in anaerobic 25 mM NaCl, pH ~ 2.5.