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. Author manuscript; available in PMC: 2009 Mar 9.
Published in final edited form as: J Biomol Screen. 2008 Jan 23;13(2):120–127. doi: 10.1177/1087057107313786

Fig. 1. Schematic diagram of the detection mechanism of ACT:One cAMP assay (BD Biosciences, Rockville, MD).

Fig. 1

The TSH receptor was cotransfected in the HEK 293 cells with a modified CNG channel, which serves as a biosensor to detect changes in the intracellular cAMP level. TSH stimulates the TSH receptor, which increases the intracellular cAMP level resulting in the activation of CNG cation channels. The membrane depolarization occurs after the influx of cations such as sodium and calcium, which can be detected by the membrane potential dye in the live cells.