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. Author manuscript; available in PMC: 2009 Mar 9.
Published in final edited form as: J Biomol Screen. 2008 Jan 23;13(2):120–127. doi: 10.1177/1087057107313786

Fig. 4. Scatter plot of the results from a 1536-well DMSO plate to establish assay performance parameters.

Fig. 4

Cells were seeded at a density of 1000 cells/well. Columns 1–4 were controls: 30 nM TSH was added to the wells in column 1 to establish maximum signal; wells in column 2 received a TSH titration ranging from 0.1 nM to 1 µM in duplicate; columns 3 and 4 received DMSO as the negative control; DMSO was added to columns 5–48. Resulting signal-to-basal (S/B) ratio was 2.5, CV was 6.3 % and Z’ factor was 0.83.