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. Author manuscript; available in PMC: 2009 Mar 9.
Published in final edited form as: J Biomol Screen. 2008 Jan 23;13(2):120–127. doi: 10.1177/1087057107313786

Fig. 5. HTS performance of 200 plates.

Fig. 5

(a). Signal-to-basal (S/B) ratio. The total signals were obtained from the wells in the columns 1 and 2 (with 30 nM TSH) and the basal signals were collected from the wells in the column 4 which received DMSO. (b) Coefficient of variation (% CV), calculated as the standard deviation (s.d.) from the wells in the column 4 divided by the s.d. from the wells in the columns 1 and 2. (c) Z’ factor, calculated by Z’ = 1- (3*s.d. of total signal + 3*s.d. of basal signal)/(total signal – basal signal) 18.