. Author manuscript; available in PMC: 2009 Mar 9.

Published in final edited form as: J Biomol Screen. 2008 Jan 23;13(2):120–127. doi: 10.1177/1087057107313786

Table 1.

A protocol for the TSH receptor cAMP assay

Step	Parameter	Value	Description
1	Cells	4 µL	1000 TSHR or parental cells/well
2	Incubation	18 – 30 hours	37°C; 5% CO₂
3	Reagent	4 µL	Membrane potential dye
4	Incubation	30 min	at room temperature
5	Compound	20 nL	Compound Libraries or control

6	Incubation	30 min	at room temperature

7	Detector	Ex = 535 and	Envision plate reader
		Em = 590 nm