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. Author manuscript; available in PMC: 2009 Mar 9.
Published in final edited form as: J Biol Chem. 2006 Sep 18;281(45):34705–34715. doi: 10.1074/jbc.M605738200

FIGURE 2. TNFR1 is located in lipid rafts prepared under different conditions.

FIGURE 2

Human airway smooth muscle cells extracted with cold Triton X-100 (A), Brij 98 at 37 °C (B), or in the absence of detergent (C) were fractionated by sucrose density gradient centrifugation. Equal volumes of the 12 collected fractions from each gradient were separated by SDS-PAGE and analyzed by immunoblotting. Lipid rafts were identified by their low buoyant density and the expression of raft proteins caveolin-1 and flotillin-1 and the raft-associated glycosphingolipid GM1. RhoGDI and the transferrin receptor (TfR) were used as markers of nonraft fractions.