Skip to main content
. Author manuscript; available in PMC: 2009 Mar 9.
Published in final edited form as: J Biol Chem. 2006 Sep 18;281(45):34705–34715. doi: 10.1074/jbc.M605738200

FIGURE 6. Caveolin-1 is not required for raft localization of TNFR1, signaling adaptors, or RhoA.

FIGURE 6

A, human airway smooth muscle cells, treated with either control or caveolin-1 siRNA (100 nm), were fractionated into soluble (S) and raft (R) fractions as described in the legend to Fig. 3. Equal volumes of cell extracts were analyzed by immunoblotting for the indicated proteins. B, HepG2 cells, which do not express caveolin-1, were extracted with cold Triton X-100 and fractionated by sucrose density gradient centrifugation. Equal volumes of soluble (S) and raft (R) fractions were separated by SDS-PAGE and analyzed for the indicated proteins by immunoblotting. C, HepG2 cells were treated with TNF-α (200 ng/ml) at 37 °C for the indicated times. Cell extracts were analyzed for the indicated proteins, and RhoA-GTP was isolated as described in the legend to Fig. 4.