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. Author manuscript; available in PMC: 2009 Mar 9.
Published in final edited form as: Dev Dyn. 2005 Nov;234(3):613–621. doi: 10.1002/dvdy.20544

Fig. 4. Hand2 activation of Mash1 and transcriptional activity is dependent on p300/CBP.

Fig. 4

The role of p300/CBP in Hand2 function was examined by inhibition of p300/CBP function with E1A. (A–C) Neuro2a cells were transfected with pEGFP-HAND2 and monitored for co-expression of Hand2 by GFP fluorescence (A), expression of Mash1 with a Mash1 specific monoclonal antibody (B) and cell number with DAPI nuclear staining (C). Mash1 expression was activated in over 80% of cells co-expressing Hand2 and eGFP. (D) Hand2 was co-expressed with the p300/CBP inhibitor protein E1A (µg of plasmid transfected). Hand2 activation of Mash1 expression was inhibited in a dose dependent manner by E1A. Co-expression of p300 rescues the inhibitory effect of E1A suggesting E1A inhibition targets the Hand2-p300/CBP complex. (E) Expression of E1A repressed the transcriptional activity of Hand2 in a dose dependent manner that was overcome by co-expression of p300.