Fig. 1.

Effect of Src kinase, PLC, and PLA₂ on parathyroid hormone (PTH) regulation of Na⁺-K⁺-ATPase-mediated ⁸⁶Rb uptake. Opossum kidney (OK) cells were treated for 15 min with 100 nM PTH in the presence or absence of the Src kinase inhibitor PP2 (100 nM; A), PLC inhibitor edelfosine (ET; 30 μM; A), the calcium-independent PLA₂ (iPLA₂) inhibitor bromoenol lactone (BEL; 1 μM; A), the cytosolic (cPLA₂) and iPLA2 inhibitor methyl arachidonyl fluorophosphonate (MAFP; 1 μM; B), or the calcium inhibitors BAPTA-AM (20 μM) or SKF-96365 (25 μM) (D). Ouabain-sensitive ⁸⁶Rb uptake (A, B, and D) or intracellular calcium (C) was measured as described in methods. Bars represent ouabain-sensitive ⁸⁶Rb uptake as means ± SE from 6 individual experiments run in triplicate (n = 6). *P < 0.05 by ANOVA followed by Bonferroni analysis.