Fig. 7.

Effect of cPLA₂ and iPLA₂ small interfering (si)RNA on PTH-mediated regulation of Na⁺-K⁺-ATPase α₁-subunit. OK cells were transiently transfected with control, cPLA₂, or iPLA₂ siRNA as described in methods. Cells were treated for 15 min with 100 nM PTH for 24 h after transfection. Cells were lysed and subjected to 10% SDS-PAGE (A and B) for Western blot analysis for phospho-ERK1/2 (A) and phospho-PKCα (B). Samples from the same lysates were analyzed for Na⁺-K⁺-ATPase α₁-subunit phosphorylation by immunoprecipitation as described above (C). A representative blot from 2 independent experiments is shown. Western blots are shown for phosphorylated ERK1/2, phospho-PKCα, and phosphoserine. Nitrocellulose membranes were stripped and reprobed for total ERK, PKCα, and Na⁺-K⁺-ATPase α₁-subunit (n = 2).