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Immunocytochemistry. HEK293-H cells expressing NBCe1-A-Q29X (A, B, and D) or wt-NBCe1-A (C). NBCe1-A-Q29X mutant: Nomarski (A) and lack of staining with NH2-terminal NBCe1-A antibody (B). C: wt-NBCe1-A is expressed on the plasma of HEK293-H cells. Treatment of the cells with G418 (75 μg/ml) restored expression and plasma membrane localization of the cotransporter (D).
