Figure 4. Knockdown of ARNTL, TNIP1 and RBCK1 prevents p21^CIP1induction in BJtsLT cells.

BJtsLT cells were infected at 32°C and shifted to 39°C for colony formation. After 14 days of culturing at 39°C cells were harvested, protein lysates were prepared and subjected to western blot for p53, CDK4 (control) and p21^CIP1 (Figure 4a–c). Additionally, total RNA was isolated and used for QRT-PCR for p21^CIP1 (Figure 4d).