Table 2. Summary of activity of compounds in inhibiting Stat3 binding to pY peptide ligand in a surface plasmon resonance binding (SPR) assay, in inhibiting IL-6-mediated Stat3 phosphorylation (pStat3) and in inhibiting IL-6-mediated Stat3 nuclear translocation in a high-throughput fluorescence microscopy (HTFM) assay.

Assay	Cpd3	Cpd30	Cpd188	Cpd3-2	Cpd3-7	Cpd30-12
SPR	4471	30	20	256	137	114
pStat3	91	18	73	144	63	60
HTFM	131	77	39	150	20	>300

¹Data presented are IC₅₀ values (µM) obtained using results summarized in Figures 1 (SPR), Figure 2 (pStat3) and Figure 4 (HTFM).