Figure 3. Effect of plate storage on assay detection.
½ log dilutions of NiV and HeV were incubated, immunolabeled and detected as described in Figure 1 (24 hours, HRP-CL). 4 plates containing 3 replicate wells were assayed immediately and compared to 4 plates stored at 4°C for 1 week prior to assay. Corresponding raw signal values (RLU, relative light units) for each virus dilution were plotted as Fresh (assayed immediately) vs Stored (assayed after storage). Correlation coefficients for each virus are indicated.