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. Author manuscript; available in PMC: 2009 Apr 1.

Published in final edited form as: J Virol Methods. 2008 Mar 4;149(1):12–19. doi: 10.1016/j.jviromet.2008.01.016

Table 1.

Summary of immunoassay combinations evaluated.

	Assay Format
	AP-CL	HRP-CL	HRP-TMB	FL
Protein block	2% skim milk	2% skim milk	2% skim milk	2% skim milk
1° antisera	α-NiV N	α-NiV N	α-NiV N	α-NiV N
Enzyme block	-	1% H₂O₂	1% H₂O₂	-
2° antisera conjugate	alkaline phosphatase¹	horse radish peroxidase³	horse radish peroxidase³	Alexa-fluor 488¹
Substrate	CDP-Star²	CPS-3³	TMB³	-

¹

Invitrogen,

²

Applied Biosystems,

³

Sigma.