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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Cell Immunol. 2008 Aug 30;254(2):94–104. doi: 10.1016/j.cellimm.2008.07.002

Figure 4. Fluorescence microscopy of BCG-GFP+ cells obtained by FACS.

Figure 4

A. Paired differential interference contrast (DIC) and fluorescence microscopy (GFP) images of cells from the R6 gate that contained BCG-GFP (panels 1–3) and one example (panel 4) of a highly autofluorescent cell with granules that represents an apparent false GFP+ event (4). Bars = 5 microns. B. Cells from gate R6 were scored by fluorescence microscopy for the number of mycobacteria/cell. Each data point represents the mean +/− SD from 3 independent experiments (50–225 cells were scored for each experiment).