Table 1.

Summary of lung cell subsets

Cell subset name	Flow cytometry gate parameters	Gate name
Total lung cells^*1	Scatter exclusion of subcellular debris	R1
CD11b or CD11c affinity-sorted cells^*2	Scatter exclusion of subcellular debris	R1
Alveolar macrophages^*3	CD11b^neg-low/CD11c^mid-high	R2
DCs^*3	CD11b^high/CD11c^high	R4
Lung macrophages^*3	CD11b^high/CD11c^neg-mid	R3
Total lung APCs	R2 + R3 + R4	R2 + R3 + R4

^*1

Used only for studies that did not assess GFP+ events. R1-gated total lung homogenate. The R1 gate contains all events except those that represent subcellular debris based on optical scatter analysis.

^*2

Used for all studies that assessed GFP+ events. Cells were derived from total lung homogenate by immunoaffinity bead sorting, and subcellular debris was excluded with the R1 gate.

^*3

Lung APC subset definitions adapted from Gonzalez-Juarrero [14]. R2, R3 and R4 events were derived from R1-gated total lung homogenate or R1-gated CD11b or CD11c affinity-sorted cells.