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Kinetic parameters for CO binding and dissociation of Fe2+ cyt c
| process | measured rate constant | Ea (kcal mol−1) | apparent rate at 20 °C (1 mM CO) |
|---|---|---|---|
| U + CO → U-CO | 1.76 ± 0.08 ×105 M−1s−1 (10 °C) | 11.2 ± 0.4 | 350 s−1 |
| U-CO → U + CO | 3.41 ± 0.15 × 10−3 s−1 (20 °C) | − | 3.4 × 10−3 s−1 |
| (N→M) + CO→M-CO | 2.0 ± 0.1 × 10−4 s−1 (20 °C) | 30.3 ± 1.8 | 2.0 × 10−4 s−1 |
| M-CO → M + CO | 3.1 ± 0.5 × 10−5 s−1 (10 °C) | 26 ± 3 | 1.5 × 10−4 s−1 |
