FIG. 7.

PUS1 mutants produce more and smaller cysts during the chronic infection. (A) Brains were harvested from infected mice at 4 wpi, and half of each brain was homogenized by passage through a 100-μm cell strainer. The homogenate was plated out, and cysts were counted only if they were labeled with the bradyzoite-specific cyst wall marker rhodamine-conjugated Dolichos bifloros agglutinin and expressed parasite-derived GFP. The results for the brains of three mice are represented as scatter plots for the mean cyst number. *, P < 0.05. (B) Images of the first seven cysts from each mouse analyzed at 4 wpi were captured, for a total of 21 images for each strain. The diameter of each cyst was measured using the obtained images, and results are represented as a scatter plot of the mean cyst diameter. (C) Cyst counts at 9 wpi. Details are as described for panel A. **, P ≤ 0.01. (D) Cyst size at 9 wpi. Details are as described for panel B. **, P ≤ 0.01.