TABLE 3.
Oligonucleotide primers for analysis of cell wall gene repeat regions
| Primera | Target | Oligonucleotide sequence (5′→3′) |
|---|---|---|
| ALS1RFw | ALS1 tandem repeat region | GATTCAATTGACACAGTG |
| ALS1RRv | ALS1 tandem repeat region | TCTTTGAACTTGACATGC |
| ALS2RFw | ALS2 tandem repeat region | GTCATTGTTGCTACAACCCG |
| ALS2RRv | ALS2 tandem repeat region | ATGACACTATTGGTGCCACC |
| ALS3RFw | ALS3 tandem repeat region | CTGCTACAGATGTTAATTCG |
| ALS3RRv | ALS3 tandem repeat region | GTTGGTGTAATGAGGACGAG |
| ALS4RFw | ALS4 tandem repeat region | CCAGTGAATGGACAGGAACA |
| ALS4RRv | ALS4 tandem repeat region | ATTGCCACGCTTGTTTTACC |
| ALS5RFw | ALS5 tandem repeat region | TCCACTTCCAAATCCAAC |
| ALS5RRv | ALS5 tandem repeat region | TATACTTGATGACTGCTC |
| ALS6RFw | ALS6 tandem repeat region | GTTGTACAAGTTCCACTG |
| ALS6RRv | ALS6 tandem repeat region | TATACTTGAAGTCTCGTC |
| ALS7RFw | ALS7 tandem repeat region | CAGCGACAACCACTTACTTCG |
| ALS7RRv | ALS7 tandem repeat region | CCCAATTGAGCTTGATGGAA |
| ALS9RFw | ALS9 tandem repeat region | TGACGGGGATGTGATCGTAG |
| ALS9RRv | ALS9 tandem repeat region | ATCCAGTTCCGAGTGCAGGT |
| HYR1RFw | HYR1 tandem repeat region | GGAGGTAATGAAAGTGGTTC |
| HYR1RRv | HYR1 tandem repeat region | CCTTCATTAGAACCAGAGTG |
| HYR3R1Fw | HYR3 tandem repeat region 1 | TCAGTTAGTGCTTCAGCAGC |
| HYR3R1Rv | HYR3 tandem repeat region 1 | CTCTTACATACAACAGGAGG |
| HYR3R2Fw | HYR3 tandem repeat region 2 | AGTCTTGAAACACCTGTCCC |
| HYR3R2Rv | HYR3 tandem repeat region 2 | TGATGGAACAACCGAGAGTG |
a
Forward primers and reverse primers are indicated by Fw and Rv suffixes, respectively, at the end of the primer name.