TABLE 4.
Isolatea | Virulence rank by:
|
Sum of virulence ranks | Strain characters
|
Growth rate [μmax (h−1)] in RPMI 1640 at:
|
Physiological tests in vitro
|
Adherence tests in vitrof
|
||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
MST | Log10 CFU/g kidney | Wt change (day 0 to day 3) | Clade | ABC type | MAT type | 30°C | 37°C | 40°C | Biofilm formation (supernatant OD600) | Growth (OD600) in YCB-BSAe at 30°C | Acid phosphatase sp act (nmol/15 min/OD600) | Alcian blue stain (μg bound per cell OD600) | Growth in 2 M NaCl | BECs with Cal (%) | Cal/BEC | Catheter plasticg | ||
J990102b | 2 | 5 | 6 | 13 | 3 | B | a/α | 0.18 | 0.23 | 0.18 | 0.07 | 0.09 | 388 | 87 | − | 34 | 2.3 | 1.2 |
YsU751b | 6 | 1 | 7 | 14 | 1 | A | a/α | 0.17 | 0.23 | 0.11 | 0.02 | 0.07 | 672 | 112 | + | 27 | 1.9 | 1.4 |
AM2002/0087b | 12 | 2 | 4 | 18 | 1 | A | a/α | 0.17 | 0.23 | 0.13 | 0.03 | 1.41 | 210 | 92 | + | 38 | 1.6 | 1.0 |
J981318b | 3 | 13 | 3 | 19 | 1 | A | a/α | 0.11 | 0.24 | 0.16 | 0.02 | 0.11 | 319 | 79 | + | 31 | 2.3 | 1.4 |
J951361b | 1 | 20 | 2 | 23 | 1 | A | a/α | 0.17 | 0.20 | 0.14 | 0.01 | 0.42 | 232 | 72 | − | 30 | 1.7 | 1.4 |
L1086b | 8 | 3 | 13 | 24 | 4 | B | a/α | 0.16 | 0.23 | 0.18 | 0.04 | 1.45 | 512 | 114 | − | 26 | 1.3 | 1.3 |
AM2003-020b | 13 | 6 | 9 | 28 | 4 | C | a/α | 0.16 | 0.29 | 0.14 | 0.03 | 0.16 | 296 | 108 | + | 27 | 1.6 | 1.7 |
IHEM16614b | 5 | 15 | 8 | 28 | 2 | A | a/α | 0.15 | 0.22 | 0.17 | 0.03 | 0.20 | 149 | 131 | − | 21 | 1.8 | 1.6 |
SC5314b | 4 | 24 | 1 | 29 | 1 | A | a/α | 0.15 | 0.27 | 0.18 | 0.00 | 0.11 | 530 | 76 | + | 27 | 2.0 | 1.3 |
AM2003/0191b | 7 | 14 | 10 | 31 | 2 | A | a/α | 0.16 | 0.21 | 0.16 | 0.05 | 0.61 | 270 | 122 | + | 35 | 2.5 | 1.1 |
s20152.082b | 19 | 4 | 12 | 35 | 4 | C | a/α | 0.17 | 0.23 | 0.18 | 0.05 | 0.12 | 436 | 123 | − | 25 | 2.1 | 1.7 |
IHEM16972b | 9 | 18 | 16 | 43 | 2 | A | a/α | 0.17 | 0.21 | 0.16 | 0.02 | 0.28 | 192 | 70 | − | 22 | 1.8 | 1.0 |
FJ9b | 22 | 7 | 15 | 44 | 1 | A | a/α | 0.17 | 0.16 | 0.12 | 0.03 | 0.36 | 610 | 72 | + | 35 | 2.1 | 1.1 |
s20152.013b | 23 | 16 | 5 | 44 | 4 | A | a/α | 0.16 | 0.27 | 0.16 | 0.02 | 0.12 | 320 | 84 | − | 27 | 2.1 | 1.0 |
73/025c | 18 | 9 | 19 | 46 | 3 | B | a/α | 0.17 | 0.19 | 0.13 | 0.05 | 0.07 | 509 | 78 | − | 39 | 2.1 | 1.2 |
AM2003-013c | 14 | 17 | 17 | 48 | 1 | A | a/α | 0.18 | 0.25 | 0.11 | 0.02 | 0.09 | 832 | 101 | + | 28 | 2.5 | 1.1 |
FC22c | 17 | 8 | 23 | 48 | 2 | A | a/α | 0.18 | 0.23 | 0.16 | 0.06 | 0.64 | 432 | 144 | − | 24 | 1.6 | 1.3 |
s20122.073c | 10 | 27 | 11 | 48 | 4 | B | a/α | 0.06 | 0.13 | 0.07 | 0.12 | 0.06 | 624 | 111 | − | 36 | 2.7 | 1.1 |
T101c | 16 | 19 | 21 | 56 | 3 | B | a/a | 0.11 | 0.15 | 0.10 | 0.02 | 1.37 | 680 | 82 | − | 39 | 2.5 | 1.1 |
s20175.016c | 35 | 10 | 14 | 59 | 4 | C | a/α | 0.13 | 0.23 | 0.11 | 0.25 | 0.11 | 957 | 107 | − | 16 | 1.8 | 0.8 |
J981315c | 15 | 29 | 18 | 62 | 3 | B | a/α | 0.18 | 0.28 | 0.15 | 0.05 | 0.10 | 455 | 88 | − | 34 | 2.0 | 1.5 |
IHEM16945c | 20 | 21 | 24 | 65 | 2 | A | α/α | 0.15 | 0.18 | 0.15 | 0.00 | 0.53 | 231 | 134 | − | 26 | 2.0 | 1.3 |
AM2003/0182c | 28 | 12 | 30 | 70 | 2 | A | a/α | 0.15 | 0.21 | 0.16 | 0.02 | 0.10 | 442 | 110 | + | 36 | 2.0 | 1.2 |
AM2003/0100c | 25 | 28 | 22 | 75 | 2 | A | a/α | 0.12 | 0.13 | 0.12 | 0.03 | 0.43 | 144 | 61 | − | 33 | 2.5 | 1.5 |
AM2004/0028c | 24 | 22 | 29 | 75 | 2 | A | a/α | 0.15 | 0.19 | 0.12 | 0.02 | 0.08 | 365 | 97 | − | 29 | 1.9 | 0.7 |
J981301c | 37 | 11 | 28 | 76 | 1 | A | a/α | 0.18 | 0.26 | 0.11 | 0.01 | 0.08 | 387 | 80 | + | 30 | 2.3 | 0.6 |
s20176.079c | 26 | 26 | 27 | 79 | 4 | A | a/α | 0.16 | 0.20 | 0.11 | 0.03 | 0.06 | 273 | 108 | + | 20 | 1.5 | 1.4 |
AM2003-016c | 31 | 32 | 20 | 83 | 1 | A | a/α | 0.16 | 0.25 | 0.11 | 0.01 | 0.09 | 596 | 78 | + | 30 | 2.3 | 1.5 |
AM2003/0089c | 21 | 37 | 26 | 84 | 2 | A | a/α | 0.16 | 0.20 | 0.14 | 0.01 | 0.32 | 124 | 79 | + | 21 | 1.7 | 1.2 |
b30708/5d | 11 | 31 | 43 | 85 | 3 | B | a/α | 0.18 | 0.24 | 0.13 | 0.07 | 0.10 | 539 | 92 | − | 34 | 1.5 | 1.5 |
HUN68d | 30 | 23 | 34 | 87 | 4 | B | a/α | 0.16 | 0.24 | 0.15 | 0.02 | 0.32 | 582 | 109 | − | 23 | 1.6 | 1.8 |
HUN92d | 29 | 40 | 25 | 94 | 1 | A | a/α | 0.12 | 0.30 | 0.07 | 0.06 | 0.07 | 701 | 112 | + | 21 | 2.1 | 1.4 |
AM2003/0165d | 27 | 38 | 32 | 97 | 3 | B | α/α | 0.12 | 0.13 | 0.16 | 0.00 | 0.11 | 1049 | 73 | + | 35 | 3.1 | 1.3 |
AM2003/0074d | 38 | 25 | 35 | 98 | 3 | B | a/α | 0.15 | 0.28 | 0.10 | 0.02 | 0.10 | 703 | 85 | − | 20 | 1.4 | 0.9 |
AM2003/0069d | 32 | 36 | 33 | 101 | 2 | A | a/α | 0.17 | 0.22 | 0.14 | 0.02 | 0.40 | 143 | 128 | + | 26 | 2.1 | 1.0 |
RV4688d | 43 | 33 | 31 | 107 | 4 | C | a/α | 0.14 | 0.27 | 0.14 | 0.73 | 0.38 | 1261 | 77 | − | 25 | 1.6 | 1.4 |
RIHO9d | 34 | 34 | 41 | 109 | 1 | A | a/a | 0.11 | 0.23 | 0.13 | 0.01 | 0.12 | 2060 | 86 | + | 33 | 2.0 | 1.0 |
AM2005/0377d | 40 | 30 | 40 | 110 | 4 | B | a/a | 0.13 | 0.13 | 0.05 | 0.00 | 0.85 | 542 | 27 | − | 31 | 2.3 | 1.2 |
81/064d | 33 | 39 | 39 | 111 | 1 | A | α/α | 0.16 | 0.21 | 0.14 | 0.01 | 0.02 | 388 | 145 | + | 26 | 1.9 | 1.3 |
FC28d | 36 | 41 | 36 | 113 | 3 | B | α/α | 0.17 | 0.24 | 0.11 | 0.02 | 0.18 | 504 | 81 | − | 42 | 2.0 | 1.2 |
IHEM3742d | 42 | 35 | 38 | 115 | 4 | C | a/α | 0.13 | 0.22 | 0.13 | 0.83 | 0.43 | 1238 | 74 | − | 28 | 1.8 | 0.3 |
HUN96d | 41 | 42 | 37 | 120 | 1 | A | a/α | 0.12 | 0.20 | 0.16 | 0.01 | 0.07 | 2522 | 95 | + | 24 | 2.1 | 1.2 |
78/028d | 39 | 43 | 42 | 124 | 1 | A | a/α | 0.13 | 0.20 | 0.09 | 0.00 | 1.32 | 225 | 111 | + | 20 | 1.5 | 0.9 |
Isolates are listed in decreasing order of virulence, as determined by the sum of virulence ranks (fifth column from the right).
Isolates ranked as highly virulent.
Isolates ranked as intermediate in virulence.
Isolates ranked as low virulence.
YCB-BSA, yeast carbon base with bovine serum albumin.
Cal, C. albicans.
% reduction Alamar blue per g catheter.