TABLE 5.
Strain property or test result | Value for isolates in:
|
|||
---|---|---|---|---|
Clade 1 (n = 14) | Clade 2 (n = 10) | Clade 3 (n = 8) | Clade 4 (n = 11) | |
Type A,a no. of isolates (% of clade) | 14 (100) | 10 (100) | 0 (0) | 2 (18) |
Type B,a no. of isolates (% of clade) | 0 (0) | 0 (0) | 8 (100) | 4 (36) |
Type C,a no. of isolates (% of clade) | 0 (0) | 0 (0) | 0 (0) | 5 (45) |
μmax (mean ± SEM) (h−1) in RPMI 1640 at the following temp: | ||||
30°C | 0.15 ± 0.01 | 0.16 ± 0.01 | 0.16 ± 0.01 | 0.14 ± 0.01 |
37°C | 0.23 ± 0.01 | 0.20 ± 0.01 | 0.22 ± 0.02 | 0.22 ± 0.02 |
40°C | 0.13 ± 0.01 | 0.15 ± 0.01 | 0.13 ± 0.01 | 0.13 ± 0.01 |
Biofilm formation (mean ± SEM) (supernatant OD600) | 0.017 ± 0.004 | 0.027 ± 0.006 | 0.037 ± 0.030 | 0.191 ± 0.095 |
Growth (mean ± SEM) (OD600) in YCB-BSAb at 30°C | 0.13 ± 0.13 | 0.36 ± 0.07 | 0.27 ± 0.17 | 0.37 ± 0.14 |
Acid phosphatase sp acta (mean ± SEM) (nmol/15 min/OD600) | 740 ± 190 | 250 ± 40 | 600 ± 80 | 640 ± 110 |
Alcian blue (mean ± SEM) (μg bound per cell OD600) | 94 ± 6 | 110 ± 10 | 80 ± 2 | 90 ± 9 |
Growth in 2 M NaCl,a no. of isolates (% of clade) | 13 (93) | 4 (40) | 1 (13) | 2 (18) |
No. of BECs positive for C. albicans (mean ± SEM) | 28 ± 1.4 | 27 ± 1.9 | 35 ± 2.5 | 26 ± 1.6 |
No. of C. albicans per BEC (mean ± SEM) | 2.0 ± 0.1 | 2.0 ± 0.1 | 2.1 ± 0.2 | 1.8 ± 0.1 |
Adherence to catheter plastic (mean ± SEM) (% reduction Alamar blue per g catheter) | 1.2 ± 0.1 | 1.2 ± 0.1 | 1.3 ± 0.1 | 1.2 ± 0.1 |
These properties were significantly different between clades (Kruskall-Wallis test, P < 0.01).
YCB-BSA, yeast carbon base with bovine serum albumin.