FIG. 3.

Conidia of the ΔpkaR mutant are hypersensitive to oxidative and nutritional stress. (A) Conidia were treated with H₂O₂ upon harvesting or after 4 h of incubation in YG. Conidia were then stained with FUN-1 to determine viability (n > 100). Statistical significance was determined by chi-square analysis (P < 0.01). (B) Conidia were harvested and diluted to a concentration of 1.5 × 10³/ml. A 150-μl aliquot was then plated onto AMM at the time of harvesting (day 0) or after incubation at 4°C for 24 h (day 1) or 7 days (day 7). CFU were counted after 2 days of incubation at 37°C and are displayed as a percentage of CFU at day 0.