FIG. 5.

Genes involved in the cell cycle are upregulated in the ΔpkaR mutant. Conidia of the wt or the ΔpkaR mutant were incubated in YG at 37°C, and total RNA was extracted from cultures that corresponded to various developmental time points (swollen conidia, germlings, and hyphae). RT-PCR was performed for cell cycle genes (cln1, pcl1, and nimA) and for gpdA as a control. Reaction mixtures (50 μl) were divided into two portions. (A) PCR products are displayed on an ethidium bromide gel. (B) PCR products were stained with Sybr green I (Molecular Probes), and band intensities were determined with a phosphorimager. Expression levels for cln1, pcl1, and nimA are presented as ratios to gpdA levels for the respective strain and time point.