The methylation status of predicted MP and predicted MR CpG islands was assessed by MSP in a panel of 5 paired normal and cancerous primary lung samples. CpG islands exhibiting hypermethylation in this sample set were further tested in 15 additional normal-tumor (N-T) pairs. A, Representative MSP data for 3 predicted MP CpG islands (ERG, PP2447, SIM2) and 3 predicted MR CpG islands (TPST2, C21orf33, CRYZL1/ITSN1) in 5 N-T pairs. U, unmethylated; M, methylated. B, Summary of methylation frequencies of all CpG islands tested.