Figure 1. IFN-γ-Producing BAC Subpopulations.

BAC (2 × 10⁶) were stimulated with 10μg PPD or with M.tb H37Ra (MOI 10) for 12hrs in presence of Brefeldin A, and subsequently surface-stained with PerCp (CD3, CD4, CD8) and FITC (CD4, CD8, CD56, γδ TCR, CD45RO)-labeled anti-human monoclonal antibodies. Intracellular staining was done using an anti-human IFN-γPE monoclonal antibody. IFN-γ-producing cells were assessed in CD3⁺ vs. SSC (A-D, F) or FSC vs. SSC (E) gates using Cell Quest software. Panels (A) – (F) represent median percentages (horizontal black and white lines) and 25 and 75 percentiles (vertical bars) of IFN-γ-producing BAC subpopulations. Panel (A) CD4⁺ [CC n=10, TB n=7]; (B) CD8⁺, [CC n=10, TB n=7]; (C) γδ TCR⁺ [CC n=8, TB n=7]; (D) CD56⁺ [CC n=8, TB n=7], (E) CD3⁻CD56⁺.[CC n=8, TB n=7] and (F) memory (CD45RO⁺) CD4⁺ and CD8⁺ [CC n=4, TB n= 4]. Significance: *p ≤ 0.05, CC vs. TB (Mann-Whitney U test).