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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Tuberculosis (Edinb). 2008 Oct 9;89(1):38–47. doi: 10.1016/j.tube.2008.08.002

TABLE 1. Cell Subpopulations in BAC and PBMC.

BAC or PBMC (2 × 105 each) were surface stained with PerCp (CD3), and FITC (CD4, CD8, CD56, γδ TCR)-labeled anti-human monoclonal antibodies and analyzed by FACS analysis. Lymphocytes in BAC and PBMC were assessed in a CD3+ vs. SSC gate and NK cells in a FSC vs. SSC gate using Cell Quest software. Except for BAC numbers in BALF, values represent median percentages of CD3+ T cells or NK cells within BAC and PBMC (bold numbers) and 25 and 75 percentiles (in parentheses).

CC (n=13) TB (n=8)
BAC PBMC BAC PBMC
# BAC/10 ml BALF [106] 1.4 (0.9–1.8) - 2.3 (1.4–2.7) -
% CD3+ BAC 5 (2–15) * - 15 (8–26) -
% CD3+ CD4+ 48 (45–51)* 59 (56–66) ** 70 (57–72) 62 (53–67)
% CD3+ CD8+ 27 (17–38) 23 (15–27) 20 (15–28) 26 (18–27)
% CD3+ CD56+ 10 (6–13) 3 (2–8) 7 (5–9) 7 (5–8)
% CD3 CD56+ 4 (3–6) 14 (10–21) ** 3 (3–4) 16 (13–20) **
% CD3+ γδ TCR+ 7 (3–10) * 3 (2–5) ** 5 (4–7) 5 (4–7)
*

Significances: p ≤0.05, CC vs. TB in BAC

**

p ≤0.05, BAC vs. autologous PBMC (Mann-Whitney U test).