TABLE 1. Cell Subpopulations in BAC and PBMC.
BAC or PBMC (2 × 105 each) were surface stained with PerCp (CD3), and FITC (CD4, CD8, CD56, γδ TCR)-labeled anti-human monoclonal antibodies and analyzed by FACS analysis. Lymphocytes in BAC and PBMC were assessed in a CD3+ vs. SSC gate and NK cells in a FSC vs. SSC gate using Cell Quest software. Except for BAC numbers in BALF, values represent median percentages of CD3+ T cells or NK cells within BAC and PBMC (bold numbers) and 25 and 75 percentiles (in parentheses).
CC (n=13) | TB (n=8) | |||
---|---|---|---|---|
BAC | PBMC | BAC | PBMC | |
# BAC/10 ml BALF [106] | 1.4 (0.9–1.8) | - | 2.3 (1.4–2.7) | - |
% CD3+ BAC | 5 (2–15) * | - | 15 (8–26) | - |
% CD3+ CD4+ | 48 (45–51)* | 59 (56–66) ** | 70 (57–72) | 62 (53–67) |
% CD3+ CD8+ | 27 (17–38) | 23 (15–27) | 20 (15–28) | 26 (18–27) |
% CD3+ CD56+ | 10 (6–13) | 3 (2–8) | 7 (5–9) | 7 (5–8) |
% CD3− CD56+ | 4 (3–6) | 14 (10–21) ** | 3 (3–4) | 16 (13–20) ** |
% CD3+ γδ TCR+ | 7 (3–10) * | 3 (2–5) ** | 5 (4–7) | 5 (4–7) |
Significances: p ≤0.05, CC vs. TB in BAC
p ≤0.05, BAC vs. autologous PBMC (Mann-Whitney U test).