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. 2009 Jan 28;15(4):441–448. doi: 10.3748/wjg.15.441

Figure 4.

Figure 4

Dose-dependent inhibitory effects of 15d-PGJ2 and 9-cis-RA on expression of VEGF. A: Three micrograms of total RNA from PANC-1 cell line was subjected to RT-PCR. The PCR products were electrophoresed on 1.5% agarose gel and visualized with ethidium bromide staining. GAPDH was used as a control. VEGF expression was detected at the expected molecules sizes (382 bp). Lanes 1, 7, 13: size marker; lanes 2-6, cells treated with 15d-PGJ2 at 0, 2.5, 5, 7.5, 10 μmol/L, respectively; lanes 8-12, cells treated with 9-cis-RA at 0, 5, 10, 15, 20 μmol/L, respectively; lanes 14-18, cells treated with combined 9-cis-RA (10 μmol/L) and 15d-PGJ2 at 0, 2.5, 5, 7.5, 10 μmol/L, respectively; B: Semi-quantitative analysis of VEGF mRNA expression in human pancreatic carcinoma PANC-1 cell line. Data on PCR products were obtained by comparing the intensity of PCR band of VEGF with that of internal control of GAPDH using genetools software.