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Immunostaining showing expression and localization of DR5 in EC9706 cells. A: Adherent cells pretreated without brefeldin A; B: Adherent cells pretreated with brefeldin A for 30 min; C: Detached cells not pretreated with brefeldin A before trypsinization; D: Detached cells pretreated with brefeldin A for 30 min before trypsinization (× 400).
