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. Author manuscript; available in PMC: 2009 Mar 9.
Published in final edited form as: Nature. 2008 Jun 11;454(7200):56–61. doi: 10.1038/nature07086

Figure 2. Post-transcriptional regulation of miR-21 biosynthesis by TGF-β.

Figure 2

a, Expression of mature miR-21 and miR-199a normalized to U6 snRNA in PASMCs stimulated with BMP4 or TGF-β (24 h; *P < 0.05; n = 3). b, Time course of pri-miR-21, pre-miR-21 or mature miR-21 expression in PASMCs on stimulation with BMP4 (upper panel) or TGF-β (lower panel). c, PASMCs pretreated with α-amanitin were stimulated with BMP4 (5 h). Expression of pri-miR-21, pre-miR-21 and mature miR-21 or ID1 is shown (*P < 0.05; n = 3). d, Relative expression of pri-miR-21, pre-miR-21 and mature miR-21 derived from increasing amounts of human miR-21 expression construct (pCMV-miR-21) transfected into 10T1/2 cells (*P < 0.05; n = 3). Error bars represent s.e.m.