Table 2.
Gene-specific primers for RT-PCR-based expression analysis of PDE1C and G(olf)
| Target gene | Sequence (5' → 3')a | Product length (base pairs) |
| Stickleback PDE1Ca | Forward: ATGGTGCATTGGTTGACTGA | 233 |
| Reverse: CTCCAGTCGTCCTTGGAGAG | ||
| Stickleback PDE1Cb1 | Forward: CAAGGGCTTCAAGGTCACAT | 152 |
| Reverse: CCTTTTCCTCCAGGTCTTCC | ||
| Stickleback PDE1Cb2 | Forward: ACAGACGGACCTCCAACATC | 238 |
| Reverse: TGTTTGCTGTAGCCCACTTG | ||
| Stickleback PDE1Cb3 | Forward: CAAAGGCTTGTCCCTGCTAC | 217 |
| Reverse: TGGTTCCACCATGAAGTCAA | ||
| Stickleback PDE1Cb4 | Forward: TGGGCTACAGCAAACACAAG | 243 |
| Reverse: AGTTCTCCAAAGCTCGGTCA | ||
| Stickleback PDE1Cb5 | Forward: CACTGGCTCAGTGAGTTGGA | 185 |
| Reverse: CTGTGCTGTAGAAGGCGACA | ||
| Stickleback PDE1Cb6 | Forward: CACTGGCTCAGTGAGTTGGA | 239 |
| Reverse: AGCTCTCTGCTCCACTCGTC | ||
| Stickleback PDE1Cb7 | Forward: CTCCTTGGAAGTGGGCTACA | 230 |
| Reverse: TGTACAACATGGCGGTGTCT | ||
| Stickleback G(olf) | Forward: SAGCAGCAGCTACAACATGG | 411 |
| Reverse: CATTCKCTGGATGATGTCMC |
a Positions with mixed bases are designated by their IUB (the International Union of Biochemistry) codes: R = A/G; Y = C/T; K = G/T; M = A/C; S = G/C; W = A/T.