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Amt Topology. Cytosolic localization of C-terminal YFP tags. The AmtA and AmtB YFP strains (green) were incubated with TRITC-dextran to label endosome lumens (red). After cell lysis, the endosomes were concentrated by centrifugation, and the resuspended pellet was left untreated or treated with proteinase K. Images are confocal with 2 μm scale bars.
