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Mutation of Eya3 causes a loss of exons 8 – 15 in homozygous mutants. (a) RT-PCR with cDNA of wild type and Eya3-/- embryos at developmental stages E11.5 (whole embryo), E13.5 (head), E15.5 (head) showed the loss of exons 8 – 15 of the Eya3 gene in Eya3-/- mice. (b) Northern-Blotting using RNA of wild-type and Eya3-/- embryos (E15.5) with a riboprobe against exons 8 – 11 confirmed the data received from RT-PCR.
