Table 1.

Secreted phospholipase A2 (sPLA2) activity of recombinant wild-type or mutated VP1-unique region (VP1u) proteins

Proteins	sPLA2 activity (μmol/min/mL)
bvPLA2 (1 ng)	0.56 ± 0.12
VP1u (0.5 ug)	0.03 ± 0.02
VP1u (5 ug)	0.19 ± 0.03 *,&
VP1uD175A(0.5 ug)	ND
VP1uD175A (5 ug)	ND
Rabbit anti-VP1u IgG (0.5 ug)	ND
Rabbit anti-VP1u IgG (5 ug)	ND
Rabbit anti-VP1uD175A IgG (0.5 ug)	ND
Rabbit anti-VP1uD175A IgG (5 ug)	ND
Rabbit IgG (0.5 ug)	ND
Rabbit IgG (5 ug)	ND
Rabbit anti-NS1 IgG (0.5 ug)	ND
Rabbit anti-NS1 IgG (5 ug)	ND
VP1u (5 ug) + Rabbit IgG (0.5 ug)	0.18 ± 0.07
VP1u (5 ug) + Rabbit IgG (5 ug)	0.19 ± 0.06
VP1u (5 ug) + Rabbit anti-B19-VP1u IgG (0.5 ug)	0.20 ± 0.11
VP1u (5 ug) + Rabbit anti-B19-VP1u IgG (5 ug)	0.09 ± 0.03 #
VP1u (5 ug) + Rabbit anti-B19-VP1uD175A IgG (0.5 ug)	0.18 ± 0.05
VP1u (5 ug) + Rabbit anti-B19-VP1uD175A IgG (5 ug)	0.16 ± 0.06
VP1u (5 ug) + Rabbit anti-B19-NS1 IgG (5 ug)	0.16 ± 0.04
VP1uD175A (5 ug) + Rabbit anti-B19-VP1u IgG (5 ug)	ND
VP1uD175A (5 ug) + Rabbit anti-B19-VP1uD175A IgG (5 ug)	ND

bvPLA2: sPLA2 from bee venom; D175A proteins encoded by genes with point mutations in the enzyme catalytic site

ND means non-detected

* and ^&indicate P < 0.05, compared to VP1u (0.5 ug) or VP1uD175A (5 ug), respectively.

^# indicates P < 0.05, compared to VP1u (5 ug).