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. 2009 Mar 10;7(3):e1000054. doi: 10.1371/journal.pbio.1000054

Figure 4. B Cells Are Not Required for Intra-Thymic MAIT Cell Development although B Cells Induce Peripheral Expansion of MAIT Cells.

Figure 4

(A) Representative HSA and TCRβ staining (upper panel) and CD8 and CD4 staining gated on mature thymocytes (lower panel) in iVα19-Jα33/Vβ6 transgenic mice in a MR1+/+ or MR1−/− RAG−/− background (left and right panel, respectively). Percentages of mature and intermediate T cells are boxed (upper panel). Representative of four mice in each group.

(B) Representative Vβ6 and CD19 staining gated on MLN lymphocytes from iVα19/Vβ6 double transgenic RAG−/− mice on a MR1+/+ (upper panels) or MR1−/− (lower panels) background without (left panels) or 14 d after MR1− or MR1+ CD3ɛ−/− splenocyte transfer. Representative of three to ten mice per group.

(C) Absolute numbers of T lymphocytes in the MLN of the different groups analyzed: MR1+/+ (filled circles) and MR1−/− (open circles) iVα19/Vβ6 double Tg RAG−/− mice, without or after MR1− or MR1+ CD3ɛ−/− splenocyte transfer. *, p < 0.021; ***, p = 0.0007.