Figure 3. Hemangioblastic, endothelial, and primitive erythrocytic potentials among EB6 subpopulations.

Colony forming abilities in unfractionated, c-Kit⁻CD41⁻, c-Kit⁺CD41⁻, c-Kit⁻CD41⁺, or c-Kit⁺CD41⁺ EB6 cells were examined in quadruplicate. (A) Blast colony-forming assays were performed. (B) Vascular endothelial progenitors (EP) were detected by the OP9 co-culture system with cytokines as previously described [20]. (C) Erythroid colonies were detected by methylcellulose colony assays. Detected erythroid colonies contained primitive erythrocytes as identified by β-H1 globin expression (data not shown). Kruskal-Wallis testing was performed for statistical analysis.