Figure 10.

Expression of BMP-7 mRNA and protein of HPMCs after adenoviral gene transfer. (A) Representative RT-PCR shows BMP-7 gene abundance with Adv-BMP-7 transfection (lanes 4 and 5) compared with negative control (lane 1), nontransfected control (lane 2), or Adv-LacZ transfection (lane 3) at 48 h after transfection. (B) Representative Western blot shows a time course of BMP-7 protein production, which is maintained in high abundance up to 7 d (D7) of Adv-BMP-7 transfection compared with control without transfection (C) or Adv-LacZ transfection.