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. 2000 Feb 4;97(4):1920–1925. doi: 10.1073/pnas.040556397

Figure 4.

Figure 4

Expression of SP11. (a) Northern blot analysis. Total RNA from anther, pistil, and leaf tissues was hybridized with an SP11–8 (coding region) probe and a BcPCP-A1 (coding region) probe. The stage numbers correspond to different bud sizes, with 1 = 0–1 mm, 2 = 1–2 mm, 3 = 2–3 mm, 4 = 3–4 mm, 5 = 4–5 mm, 6 = 5–7 mm, 7 = 7–10 mm, and 8 = open flower. EtBr, ethidium bromide staining of the gel before blotting. (b) In situ hybridization. Anther sections derived from flower buds of stages 5 and 7 were hybridized with SP11–8 and BcPCP-A1 antisense riboprobes and their sense riboprobes (negative control).