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. Author manuscript; available in PMC: 2009 Mar 10.
Published in final edited form as: Cell. 2008 Jun 13;133(6):1032–1042. doi: 10.1016/j.cell.2008.05.039

Figure 1. Overexpression of SAK Induces Centrosome Amplification and Delays Development.

Figure 1

(A and B) Immunostaining of G2 wild-type (WT) (A) and SAKOE (B) neuroblasts with the centriole marker D-PLP (left, green in merged panel), the PCM marker γ-tubulin (middle, red in merged panel), and DNA (blue in merged panel). In the WT neuroblast the two centrosomes are asymmetric (see text for details) and only one contains high levels of γ-tubulin. In the SAKOE neuroblast eight centrosomes are present and these contain varying amounts of γ-tubulin.

(C) Quantification of centrosome number in WT (white bars) and SAKOE (red bars) neuroblasts. Note that we only scored D-PLP dots as centrosomes if they also contained some PCM.

(D and E) EM micrographs of selected thin serial sections of WT (D) and SAKOE (E) neuroblast spindle poles. In the WT cell only two centrioles were detected at the spindle pole (red and yellow arrows). In this SAKOE cell three centrioles were detected at the spindle pole (red, yellow, and orange arrows).

(F) A graph showing the percentage of WT (white bars), SAKOE (red bars), DSas-4 (light gray), and DSas-4,SAKOE (dark gray) pupae that formed between the 5th and 10th day of development. More than 95% of SAKOE pupae eclosed as adults, which is similar to WT controls. Scale bar (A and B) = 10 µm; (D and E) = 0.5 µm.